1. GPCR Cell Line
G protein–coupled receptors (GPCRs) are a large family of seven transmembrane protein receptors found eukaryotes. Coupling with G proteins, these receptors are activated by binding to diverse extracellular ligands including light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters etc. The activated GPCRs further induce intracellular signal transduction pathways and, ultimately, cellular responses. GPCRs are involved in a wide variety of physiological processes and many diseases. To date, many medicinal drugs are GPCR-targeting, and there is an increasing interest in the study of discovering novel GPCR-related drug candidates.
Engineered cell lines stably overexpressing GPCRs are useful models for detection of GPCR signaling. Creative Biogene owns a team of experienced GPCR biologists working in the area of GPCR biology and drug discovery. We have generated a wide variety of GPCR stable cells with more under development. Our expert team and facilities enable us to efficiently construct a GPCR stable cell line to meet your specific needs in the process of GPCR exploration.
2. Nonhuman Primates Gene Cloning
Nonhuman primates are usually used in scientific research as animal models of human behavior and disease. Although their use in research is overshadowed by the availability of less expensive and more abundant animal models such as zebrafish and rodents with easier husbandry, these animal models can’t always provide the best translational medicine when trying to elucidate human pathologies. Nonhuman primates represent an alternative to these animal models and provide distinct advantages due to their phylogenetic proximity to humans that lends itself to greater genetic, neurological, physiological, and behavioral similarities.
Macaque species are the most common nonhuman primate model, including the rhesus macaque (Macacamulatta) and the long-tailed macaque (M. fascicularis), also known as the crab-eating or cynomolgus macaque. In macaques, population differences have been identified with regards to physiology, behavior, susceptibility to infectious disease, and toxicology. Thus, a priori genetic information on nonhuman primates used in research can aid in more informed selection of individuals for studies and better translational models.
Nevertheless, most macaque genes have not been cloned and are not available commercially. If one needs to study a gene function and a disease target in these nonhuman primates model, one would need to clone the macaque gene, which could be quite time-consuming.
3. Antisense Oligonucleotides Synthesis
Antisense oligonucleotides (ASOs) are short (usually 15–25 nt), synthetic, single-stranded DNA oligos that are designed to specifically bind to a target messenger RNA (mRNA) by Watson–Crick hybridization, inducing selective degradation of the mRNA or prohibiting translation of the selected mRNA into protein. The hybridization of the ASO and a target mRNA depends on design and chemistry modification, which may result in selective degradation by endogenous nucleases, or alternatively, inhibition of the processing and/or function of the mRNA by an occupancy-only mechanism. ASO technology provided an oligonucleotide-based approach to disrupting gene expression and has become effective tools in basic molecular biology, and proteomics research, often used for target screening and validation as well as for drug discovery.
4. Reporter gene knockin
CB provides one-stop gene knockin cell line generation service, including reporter gene knockin, tag knockin and fragment knockin. This service ranges from design of gRNA, vector construction, cell transfection and selection, to single clone sequencing and cell expansion. Our talented scientists are expert in knockin cell line generation by using CRISPR system. At CRISPR/Cas9 platformCB, you can expect to achieve with satisfying services and products. Knockin cell line, which inserted a certain fragment in a specific locus, is a useful tool for research. When a reporter gene or tag is inserted at the specific locus, this cell line can be used to study endogenous expression level of targeted gene. With the help of CRISPR/Cas9 system, it become easier to generate a custom knockin cell line. Cas9 nuclease targets to different loci of genome through sgRNA with different sequences and then the enzyme introduces double strand break. At the presence of template, DNA double strand break can be repaired via HDR pathway. By this method, promoter, tag or gene can be inserted into genome.
So much for the introduction of biotechnology in this article. Biotechnology can change our life. I hope our world will become better and better under the influence of biotechnology.